SS6 * Light – Diabetic Neuropathy * BPA – Neonatal Heart * Gelatin – Neuromuscular Metabolism * Neonicotinoids – Estrogen * Trichotillomania – Progesterone

Photobiomodulation induces antinociception, recovers structural aspects and regulates mitochondrial homeostasis in peripheral nerve of diabetic mice

Red light therapy improves pain and nerve function in an animal model of Diabetic Peripheral Neuropathy. Scientists prove the usefulness of a pretty simple light therapy protocol using non-pulsed light, this is a relatively simple protocol to replicate. You can see my article on dosing considerations here (*)

“Diabetic Peripheral Neuropathy (DPN) is a nervous disorder caused by diabetes mellitus, affecting about 50% of patients in clinical medicine. Chronic pain is one of the major and most unpleasant symptoms developed by those patients, and conventional available treatments for the neuropathy, including the associated pain, are still unsatisfactory and benefit only a small number of patients. Photobiomodulation (PBM) has been gaining clinical acceptance once it is able to promote early nerve regeneration resulting in significant improvement peripheral nerves disabilities. In this work, the effects of PBM (660 nm, 30 mW, 1.6 J/cm2, 0.28 cm2,15 sec in a continuous frequency) on treating DPN‐induced pain and nerve damage were evaluated in an experimental model of diabetic‐neuropathy induced by streptozotocin in mice. PBM induced antinociception (pain relief) in neuropathic‐pain mice that was dependent on central opioids release. After 21 consecutive applications, PBM increased nerve growth factor levels and induced structural recovery increasing mitochondrial content and regulating Parkin in the sciatic nerve of DPN‐mice. Taking together, these data provide new insights into the mechanisms involved in the effects of photobiomodulation‐therapy emphasizing its therapeutic potential in the treatment of DPN.”

Disruption of neonatal cardiomyocyte physiology following exposure to bisphenol-a

BPA is an estrogenic chemical used in plastics, it’s a cause of arrhythmias, angina, chest pain, coronary artery disease, and atherosclerosis. A new study shows it has similar effects on neonatal rodent cells.

“Recent reports have shown a link between bisphenol-a (BPA) exposure and adverse cardiovascular outcomes; although these studies have been limited to adult subjects and models. Since cardiac physiology differs significantly between the developing and adult heart, we aimed to assess the impact of BPA exposure on cardiac function, using a neonatal cardiomyocyte model….
Acute BPA exposure altered cardiomyocyte functionality, resulting in a slowed spontaneous beating rate and increased beat rate variability. BPA exposure also impaired intracellular calcium handling, resulting in diminished calcium transient amplitudes, prolonged calcium transient upstroke and duration time. Alterations in calcium handling also increased the propensity for alternans and skipped beats. Notably, the effect of BPA-treatment on calcium handling was partially reversible. Our data suggest that acute BPA exposure could precipitate secondary adverse effects on contractile performance and/or electrical alternans, both of which are dependent on intracellular calcium homeostasis.”


Vitamin C enriched gelatin supplementation before intermittent activity augments collagen synthesis
15g of gelatin and a little vitamin C doubles a biomarker collagen synthesis in reso[ponse to skipping rope. Amino-terminal propeptide of collagen I is sometimes referred to as P1NP in the literature, and some claim it’s the most reliable indicator of healthy bone metabolism. (*)

“Eight healthy male subjects completed a randomized, double-blinded, crossover-design study in which they consumed either 5 or 15 g of vitamin C–enriched gelatin or a placebo control. After the initial drink, blood was taken every 30 min to determine amino acid content in the blood. …
Supplementation with increasing amounts of gelatin increased circulating glycine, proline, hydroxyproline, and hydroxylysine, peaking 1 h after the supplement was given. Engineered ligaments treated for 6 d with serum from samples collected before or 1 h after subjects consumed a placebo or 5 or 15 g gelatin showed increased collagen content and improved mechanics. Subjects who took 15 g gelatin 1 h before exercise showed double the amino-terminal propeptide of collagen I in their blood, indicating increased collagen synthesis.

Effects of Neonicotinoid Pesticides on Promoter-Specific Aromatase (CYP19) Expression in Hs578t Breast Cancer Cells and the Role of the VEGF Pathway

Neonicotinoids are the most widely used pesticides. A cell study shows they have estrogenic properties, altering enzymes and creating a similar pattern seen in “hormone dependent” breast cancer. CYP19 is an aromatase enzyme also altered in endometrial cancer (*).

“Hs578t cells were exposed to various signaling pathway stimulants or neonicotinoids for 24 h. …
To our knowledge, we are the first to demonstrate that the normal I.4 promoter and the breast cancer-relevant PII, I.3, and I.7 promoters of CYP19 are active in these cells. …
Exposure of Hs578t cells to environmental concentrations of (neonicotinoids) imidacloprid and thiacloprid resulted in a switch in CYP19 promoter usage, involving inhibition of I.4 promoter activity and an increase of PII, I.3, and I.7 promoter-mediated CYP19expression and aromatase catalytic activity. Greater effects were seen at lower concentrations. Our results suggest that thiacloprid and imidacloprid exert their effects at least partially by inducing the MAPK 1/3 and/or PLC pathways.
CONCLUSIONS:We demonstrated in vitro that neonicotinoids may stimulate a change in CYP19 promoter usage similar to that observed in patients with hormone-dependent breast cancer.”

Salivary sex hormones in adolescent females with trichotillomania

Trichotillomania  is an impulse control disorder characterised by an urge that results in the pulling out of one’s own hair. It’s more common in females and now associated with low progesterone.

“11 adolescent girls with trichotillomania, post-menarche and not taking birth control, were examined on a variety of clinical measures. …
Lower progesterone was associated with more severe symptoms and lower levels of all hormones were associated with worse overall functioning. Adolescents with trichotillomania exhibit a range of hormone levels but that lower levels of certain hormones may have important clinical associations.”